Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1210473 | Journal of Chromatography A | 2009 | 5 Pages |
Abstract
A specific and sensitive method based on liquid chromatography-tandem mass spectrometry using atmospheric pressure chemical ionization (LC-APCI-MS/MS) has been developed for the determination of gestagens in kidney fat (medroxyprogesterone acetate, megestrol acetate and melengestrol acetate). The procedure involved a clean-up procedure with gel permeation chromatography (GPC). The analytes were analyzed by reversed-phase LC-MS/MS, in positive multiple reaction monitoring (MRM) mode, acquiring two diagnostic product ions from the chosen precursor for the unambiguous confirmation of the gestagens. The method was validated at the validation level of 1.0 ng/g. The accuracy and precision of the method were satisfactory. The decision limits CCα ranged from 0.20 to 0.22 ng/g while the detection capabilities CCβ ranged from 0.33 to 0.38 ng/g. The method proved to be sensitive and reliable and thus renders an appropriate mean for residue analysis studies.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
George Kaklamanos, Georgios Theodoridis, Themistoklis Dabalis,