| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1210533 | Journal of Chromatography A | 2006 | 5 Pages |
Abstract
A capillary column in a rapid-flow system has been developed for detecting targeted messenger RNA (mRNA) molecules. The column has a structure made of two beds-one bed of porous microbeads and one bed of microbeads with a polythymidine base sequence. The targeted eukaryotic mRNA molecules are detected by two-step hybridization (sandwich hybridization) composed of polyadenosine selection of mRNA molecules and formation of a probe-target (targeted mRNA) hybrid. The sandwich hybridization, which is accomplished within 1Â h, was tested using synthetic polydeoxynucleotides. Ten picomoles of the targeted polydeoxynucleotide were detected.
Related Topics
Physical Sciences and Engineering
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Authors
Michihiro Ohnishi,