Article ID Journal Published Year Pages File Type
1210812 Journal of Chromatography A 2008 5 Pages PDF
Abstract

There is great interest lately in the availability of analytical methods for quantification of 3-iodothyronamine from blood and tissues. To date, no validated method for determination of 3-iodothyronamine from biological matrices has been described. Detailed in this report is an LC–MS/MS method that permits accurate and reproducible quantification of pharmacological concentrations of 3-iodothyronamine from rat serum, with a 0.0008 M lower limit of quantification. Endogenous 3-iodothyronamine was observed from rodent and human serum (0.2 mL) at the method limit of detection. In summary, the LC–MS/MS method enables quantification of circulating 3-iodothyronamine to allow examination of a relationship with biological activity.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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