Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1210812 | Journal of Chromatography A | 2008 | 5 Pages |
Abstract
There is great interest lately in the availability of analytical methods for quantification of 3-iodothyronamine from blood and tissues. To date, no validated method for determination of 3-iodothyronamine from biological matrices has been described. Detailed in this report is an LC–MS/MS method that permits accurate and reproducible quantification of pharmacological concentrations of 3-iodothyronamine from rat serum, with a 0.0008 M lower limit of quantification. Endogenous 3-iodothyronamine was observed from rodent and human serum (0.2 mL) at the method limit of detection. In summary, the LC–MS/MS method enables quantification of circulating 3-iodothyronamine to allow examination of a relationship with biological activity.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Andrea E. DeBarber, Travis Geraci, Vincent P. Colasurdo, Sarah A. Hackenmueller, Thomas S. Scanlan,