Article ID Journal Published Year Pages File Type
1210939 Journal of Chromatography A 2008 8 Pages PDF
Abstract
Multi-dimensional liquid-based separation is required for fractionation and mapping of complex protein mixtures from cells. A method that has been used as the first dimension in such separations is chromatofocusing (CF), which is based on generating a pH gradient on an anion exchange column. The use of pH in the first dimension is essential where pH is a fundamental property of proteins and can provide information on post-translationally modified forms of a protein. In this work, a micro-chromatofocusing technique is introduced which can separate microgram levels of proteins from cell lysates for further analysis by LC-MS/MS. It is shown that this method can analyze 10 μg of sample and detect nearly 700-800 proteins from ovarian cancer cell line lysates.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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