Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1210939 | Journal of Chromatography A | 2008 | 8 Pages |
Abstract
Multi-dimensional liquid-based separation is required for fractionation and mapping of complex protein mixtures from cells. A method that has been used as the first dimension in such separations is chromatofocusing (CF), which is based on generating a pH gradient on an anion exchange column. The use of pH in the first dimension is essential where pH is a fundamental property of proteins and can provide information on post-translationally modified forms of a protein. In this work, a micro-chromatofocusing technique is introduced which can separate microgram levels of proteins from cell lysates for further analysis by LC-MS/MS. It is shown that this method can analyze 10 μg of sample and detect nearly 700-800 proteins from ovarian cancer cell line lysates.
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Physical Sciences and Engineering
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Analytical Chemistry
Authors
Hyeyeung Kim, David M. Lubman,