Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1211011 | Journal of Chromatography A | 2008 | 6 Pages |
Abstract
We propose a new capillary zone electrophoresis method applying short-end injection technique for the fast evaluation of methylcystosine/total cytosine ratio after acidic DNA hydrolysis. By short-end injection and by using a 100 mmol/l Tris solution titrated with 1 mol/l phosphoric acid to pH 3.75 as background electrolyte, cytosine and methylcytosine were separated with a good resolution in less than 1.5 min. Stepwise multiple linear regression with DNA methylation degree as the dependent variable and age, cysteine, homocysteine and methionine as independent variables, showed a negative association with age and that total cysteine is the most important determinant of DNA methylation.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Salvatore Sotgia, Ciriaco Carru, Flavia Franconi, Paolo Battista Fiori, Salvatorica Manca, Silvestro Pettinato, Stefania Magliona, Renzo Ginanneschi, Luca Deiana, Angelo Zinellu,