Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1211033 | Journal of Chromatography A | 2007 | 6 Pages |
Abstract
The kinetics of keto-enol tautomerism of p-hydroxyphenylpyruvic acid (pHPP) as a model of α-carbonyl compounds in aqueous solution at room temperature (25 °C) was first investigated by capillary electrophoresis with UV detection at 200 nm. The two tautomers could be separated and detected within 3 min. Since the ketonization of enolic pHPP varied with the buffer composition and buffer pH, the kinetics of pHPP was studied under different conditions, and relevant distributing fractions of enolic pHPP, ketonization rate constants and half-life were determined. In addition, β-CD played an important part in the separation of the two tautomers, thus, the interaction between pHPP and β-CD was also investigated by electrochemical techniques.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Lu Huang, Ying Huang, Yuwu Chi, Jinming Lin, Lishuang Yu, Liangjun Xu, Guonan Chen,