Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1211104 | Journal of Chromatography A | 2007 | 4 Pages |
Abstract
(â)-Epigallocatechin gallate (EGCG) was purified in one step from a green tea polyphenol (GTP) crude extract by adsorption chromatography on a Superose 12 HR 10/30 column. The mobile phase used was a mixture of acetonitrile and water with an optimum mobile phase compositions regarding purity, recovery and separation time of 78/22 (v/v). Maximum practical sample loading was 100Â mg GTP per run (corresponding to 4.2Â mg/ml Superose). An EGCG purity of 99% with recoveries in the range 60-65% was achieved in one step directly from the crude GTP extract. Full column regeneration was obtained using solvents in the following order: 0.5Â M NaOH, distilled water and 30% acetic acid.
Related Topics
Physical Sciences and Engineering
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Authors
Jun Xu, Tianwei Tan, Jan-Christer Janson,