Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1211114 | Journal of Chromatography A | 2007 | 9 Pages |
Abstract
An electrophoretically mediated microanalysis (EMMA) method has been developed for yeast alcohol dehydrogenase and quantification of reactant and product cofactors, NAD and NADH. The enzyme substrate ethanol (1% (v/v)) was added to the buffer (50Â mM borate, pH 8.8). Results are presented for parallel capillary electrophoresis with a novel miniature UV area detector, with an active pixel sensor imaging an array of two or six parallel capillaries connected via a manifold to a single output capillary in a commercial CE instrument, allowing conversions with five different yeast alcohol dehydrogenase concentrations to be quantified in a single experiment.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Pawel L. Urban, David M. Goodall, Edmund T. Bergström, Neil C. Bruce,