Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1211169 | Journal of Chromatography A | 2007 | 8 Pages |
Abstract
Standard chromatographic methods for the quantification of bacterial poly(3-hydroxyalkanoate) (PHA) proved to be inappropriate for the analysis of medium-chain-length PHA (mcl-PHA). Transesterification catalyzed by protic acids is not quantitative for mcl-PHA under common conditions due to slow reaction kinetics and formation of side-products in case of functionalized side-chains. To circumvent these limitations, an improved method for the quantification of mcl-PHA by GC-FID was developed. Boron trifluoride in methanol was successfully applied to quantitatively methanolyse different mcl-PHA (recovery >94%). This novel method is well-suited for the analysis of purified mcl-PHA as well as for mcl-PHA in biomass.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Patrick Furrer, Roland Hany, Daniel Rentsch, Andreas Grubelnik, Katinka Ruth, Sven Panke, Manfred Zinn,