Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1211422 | Journal of Chromatography A | 2006 | 5 Pages |
Abstract
The HPLC enantiomeric separation of the racemic cularinoid alkaloids N-p-methoxy-1,α-dihydroaristoyagonine (1) and 4â²,5â²-demethoxy-1,α-dihydroaristoyagonine (2) was accomplished using five chiral stationary phases (CSPs), some of them polysaccharide-derived ones. The molecular size and conjugative effect strongly affect the different enantioselectivity of the compounds 1 and 2 on the various CSPs investigated. Single enantiomers of 1 were isolated by repeated injections on an analytical HPLC column, and their circular dichroism spectra and optical rotations were measured. The cytotoxicity of the isolated enantiomers was measured on a human colon carcinoma cell line and compared with that of the racemic compound.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Salvatore Caccamese, Giovanna Scivoli, Salvatore Bianca, Juan Manuel López-Romero, Francisco Javier Ortiz-López,