Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1211457 | Journal of Chromatography A | 2006 | 7 Pages |
Abstract
HPTLC/MS by a plunger-based extraction device was shown to be an appropriate technique for quantitative planar chromatography, even in trace analysis. Reproducible extraction from silica gel phases in the lower-pg range distinguishes this technique from other approaches. Repeatability of the MS signal showed a mean RSD of 12.5% for the example of Harman, a heterocyclic aromatic amine. Analytical response within a plate and over various plates/days showed determination coefficients of 0.9915 and 0.9488, respectively. Limit of detection (LOD) by a single quadrupole was better than 40Â pg and limit of quantitation (LOQ) by a tandem mass spectrometer better than 20Â pg. LOQ/LOD obtained were of similar magnitude as reported for HPLC/MS methods, however, this order of sensitivity was shown for the first time in the field of HPTLC/electrospray ionization MS.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ute Jautz, Gertrud Morlock,