How to achieve rapid separations in counter-current chromatography

A new generation of high performance coil planet centrifuges is now making it possible to realize the enormous potential of liquid–liquid partition chromatography for the rapid and predictable scale up of separation processes. This paper uses a separation of flavonoids to demonstrate how rapid fractionations can be obtained at high flow rates with limited loss of resolution and with significant increase in throughput. Furthermore, it is shown that chromatograms at various flows can be modeled so that optimum conditions can be rapidly assessed.