| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1211558 | Journal of Chromatography A | 2006 | 10 Pages |
Abstract
The selectivity of size-exclusion chromatography (SEC) can be modified by adding non-ionic micelles to the mobile phase. Surfactant-aided size-exclusion chromatography (SASEC) can therefore very well be performed in a gradient mode on an SMB, as is reported in this paper. A method has been developed for correctly positioning a micellar gradient over an SMB. The method is applied for size-exclusion chromatography with the non-ionic surfactant C12E23 as gradient forming solute, and demonstrated by applying it to a relevant chromatographic protein separation problem.
Related Topics
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Authors
D.A. Horneman, M. Ottens, J.T.F. Keurentjes, L.A.M. van der Wielen,