| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1211642 | Journal of Chromatography A | 2006 | 9 Pages |
Abstract
A comprehensive, fully automated strategy is demonstrated for HPLC-UV chromatographic method development using ChromSword® optimization software. The strategy involves: (1) the automated screening of various column and mobile phase combinations, (2) rational selection of the best starting conditions; and (3) subsequent automated method development to generate optimized separation methods. Pharmaceutical compounds were applied to solve problematic drug impurity separations. ChromSword® software automates the screening, optimization, and documentation steps thus reducing the method development time. The strategy was compared to a manual method development approach showing the automated method strategy affords better selectivity in a shorter time.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Elizabeth F. Hewitt, Patrick Lukulay, Sergey Galushko,