| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1211657 | Journal of Chromatography A | 2006 | 4 Pages |
Abstract
A method for quantification of the fine particle dose of lactose is described, using a hydrophilic interaction chromatography (HILIC) method and evaporative light scattering detection. The HILIC method used an aminopropyl column and a mobile phase consisting of acetonitril/water (80/20, v/v) for isocratic elution. Sensitive chromatography was obtained using a low concentration of water in the extraction solvent. The detection limit (RSD < 10%) at an injection volume of 10 μL is 10 μg/mL. Linearity was obtained in the range of 10–80 μg/mL (R2 > 0.99). A relative standard deviation (RSD) of 0.5% (N = 6) demonstrated good precision of the optimized method.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Bianca Beilmann, Peter Langguth, Heribert Häusler, Peter Grass,