Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1211687 | Journal of Chromatography A | 2006 | 8 Pages |
Abstract
The present paper reports on the separation of a mixture of fluorescein isothiocyanate-labeled angiotensin I and II peptides in a shear-driven nanochannel with a C18-coating and using an eluent consisting of 5% acetonitrile in 0.02 M aqueous phosphate buffer at pH 6.5. The flat-rectangular nanochannel in fused silica consisted of an etched structure in combination with a flat moving wall. The very fast separation kinetics that can be achieved in a nanochannel allowed to separate the angiotensin peptides in less then 0.2 s in a distance of only 1.8 mm. Plate heights as small as 0.4 μm were calculated after substraction of the injection effect.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Sarah Vankrunkelsven, David Clicq, Deirdre Cabooter, Wim De Malsche, J.G.E. Gardeniers, Gert Desmet,