| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1211699 | Journal of Chromatography A | 2006 | 8 Pages |
Abstract
Our previously reported method for the fractionation of apple procyanidins was modified successfully to achieve the separation of (epi)catechins and procyanidins (ranging from dimers to octamers) according to the degree of polymerization. Normal-phase chromatography was employed, using a hexane–methanol–ethyl acetate mixture as the mobile phase. Each fraction was characterized using high-performance liquid-chromatography electrospray-ionization mass spectrometry (HPLC-ESI/MS) and matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF/MS). This method will be useful for the evaluation of the physiological functions of proanthocyanidins and for the elucidation of their individual structures.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Toshihiko Shoji, Saeko Masumoto, Nina Moriichi, Tomomasa Kanda, Yasuyuki Ohtake,