Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1211843 | Journal of Chromatography B | 2016 | 9 Pages |
•Green DES systems were used as extraction solvent.•Macroporous resin was attempted for separation of isoflavonoids from DES solution.•Deep eutectic solvent-based negative pressure cavitation extraction was developed.•Extraction and preparative separation of isoflavonoids from D. odorifera leaves.
In this study, green and efficient deep eutectic solvent-based negative pressure cavitation-assisted extraction (DES-NPCE) followed by macroporous resin column chromatography was developed to extract and separate four main isoflavonoids, i.e. prunetin, tectorigenin, genistein and biochanin A from Dalbergia odorifera T. Chen leaves. The extraction procedure was optimized systematically by single-factor experiments and a Box-Behnken experimental design combined with response surface methodology. The maximum extraction yields of prunetin, tectorigenin, genistein and biochanin A reached 1.204, 1.057, 0.911 and 2.448 mg/g dry weight, respectively. Moreover, the direct enrichment and separation of four isoflavonoids in DES extraction solution was successfully achieved by macroporous resin AB-8 with recovery yields of more than 80%. The present study provides a convenient and efficient method for the green extraction and preparative separation of active compounds from plants.