| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1211872 | Journal of Chromatography B | 2016 | 5 Pages |
•Successful trouble-shooting for an interesting and unusual stability case.•Analyte instability in neat solutions only at high concentrations, not at lower ones.•Stable-isotope labeled internal standard did not have the similar stability issue.•Generally applicable insights provided for avoiding neat solution stability issues.
This paper presents the trouble-shooting for a very unusual stability case. Tetracaine was found unstable in neat solutions only at high concentrations, but not at low concentrations. Moreover, its stable-isotope labeled internal standard did not show similar behavior. A series of trouble-shooting experiments were conducted to uncover the root cause. Some generally applicable precautions/insights can be drawn from this investigation to avoid potential stability issues during bioanalytical method development and validation.
