| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1212076 | Journal of Chromatography B | 2015 | 5 Pages |
•Novel UPLC–MS/MS method for quantification of songorine in rat plasma.•First PK studies of songorine by intravenous administration in rats.•The run time was 3.0 min with no carryover.
An ultra performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) was developed and validated for the determination and pharmacokinetic investigation of songorine in rat plasma. Sample preparation was accomplished through a simple one-step deproteinization procedure with 0.2 mL of acetonitrile to a 0.1 mL plasma sample. Plasma samples were separated by UPLC on an Acquity UPLC BEH C18 column using a mobile phase consisting of acetonitrile-0.1% formic acid in water with gradient elution. The total run time was 3.0 min and the elution of songorine was at 1.68 min. The detection was performed on a triple quadrupole tandem mass spectrometer equipped with positive-ion electrospray ionization (ESI) by multiple reaction monitoring (MRM) of the transitions at m/z 358.3 → 340.3 for songorine and m/z 237.2 → 194.3 for carbamazepine (internal standard). The calibration curve was linear over the range of 1–1000 ng/mL with a lower limit of quantitation (LLOQ) of 1.0 ng/mL. Mean recovery of songorine in plasma was in the range of 75.2–87.5%. The intra- and inter-day precision (RSD) was between 3.1–8.5% and 4.3–9.6% and the intra- and inter-day accuracy (RE) ranged from −4.0 to 8.9% and −9.0 to 6.7%. This method was successfully applied in pharmacokinetic study after intravenous administration of 5.0 mg/kg songorine in rats.
