| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1212149 | Journal of Chromatography B | 2015 | 5 Pages |
•We determined the L/S ratio by cation-exchange resin and mass spectrometry.•The L/S ratio measured by mass spectrometry offers an alternative method to TLC.•Our method provides a faster, simpler, and reliable result of fetal lung maturity.
Fetal lung maturity is estimated using the lecithin/sphingomyelin ratio (L/S ratio) in amniotic fluid and it is commonly measured with thin-layer chromatography (TLC). The TLC method is time consuming and technically difficult; however, it is widely used because there is no alternative. We evaluated a novel method for measuring the L/S ratio, which involves a tip-column with a cation-exchange resin and mass spectrometry. Phospholipids in the amniotic fluid were extracted using methanol and chloroform. Choline-containing phospholipids such as lecithin and sphingomyelin were purified by passing them through the tip-column. LC–MS/MS and MALDI-TOF were used to directly analyze the purified samples. The L/S ratio by mass spectrometry was calculated from the sum peak intensity of the six lecithin, and that of sphingomyelin 34:1. In 20 samples, the L/S ratio determined with TLC was significantly correlated with that obtained by LC–MS/MS and MALDI-TOF. There was a 100% concordance between the L/S ratio by TLC and that by LC–MS/MS (kappa value = 1.0). The concordance between the L/S ratio by TLC and that by MALDI-TOF was also 100% (kappa value = 1.0). Our method provides a faster, simpler, and more reliable assessment of fetal lung maturity. The L/S ratio measured by LC–MS/MS and MALDI-TOF offers a compelling alternative method to traditional TLC.
