| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1212207 | Journal of Chromatography B | 2015 | 5 Pages |
•Solasonine was a potential anti-cancer agent.•A sensitive and rapid method for analysis of solasonine in rat plasma is firstly proposed.•It is the first pharmacokinetic and bioavailability study of solasonine in rats.•A simple protein precipitation was used for pretreatment and the LOD was 0.1 ng/mL.
Solasonine, a known glycoalkaloid, is a potential anti-cancer agent. In this work, a simple, sensitive and fast ultra performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the quantitative determination of solasonine in rat plasma. Plasma samples were processed with a protein precipitation. The separation was achieved by an ACQUITY HSS T3 (100 × 2.1 mm, 1.8 μm) column with a gradient mobile phase consisting of 0.1% formic acid and acetonitrile. Detection was carried out using positive-ion electrospray tandem mass spectrometry via multiple reaction monitoring (MRM). The validated method had an excellent linearity in the range of 0.1–500 ng/mL (R2 > 0.994) with a low limit of detection (0.1 ng/mL) and lower limit of quantification (0.5 ng/mL). The extraction recovery was in the range of 92.4–94.9% for solasonine and 91.9% for dendrobine (internal standard, IS). The intra- and inter-day precision was below 9.8% and accuracy was from 86.0% to 94.3%. No notable matrix effect and astaticism was observed for solasonine. The method has been successfully applied to a pharmacokinetic and bioavailability study of solasonine in rats for the first time, which provides the basis for the further development and application of solasonine.
