Article ID Journal Published Year Pages File Type
1212286 Journal of Chromatography B 2015 7 Pages PDF
Abstract

•UPLC–MS/MS method for targeted bile acids profile and quantitation.•Quantitation of unique sea lamprey bile acids in plasma and different tissues.•Contribution to the first discovery of de novo synthesis of BAs in the sea lamprey intestine.

Bile acids (BAs) have recently gained more attention because of their diverse roles from digestion to signaling. Simultaneous analyses of various BAs in biological samples are challenging due to their structural similarity, relatively low concentrations, and the presence of isomeric forms. In this study, we report a simple and sensitive UPLC–MS/MS method for simultaneous quantifications of 13 BAs including four unique sea lamprey BAs in sea lamprey plasma, liver, intestine, and gills. A straightforward protein precipitation (PPT) method was used to extract BAs from the biological samples. Separation of all target analytes was achieved on a reverse-phase UPLC column in 15 min, and detection was carried out on MS/MS with ESI in the negative ionization mode. This method was validated regarding its linearity, limits of detection (LOD), recovery, matrix effect, reproducibility, accuracy and precision. Significant improvements compared to previous LC–MS/MS methods were observed as a result of the application of UPLC and extensive optimization of experimental conditions. The method showed excellent linearity with high regression coefficients (>0.99) over a range of 0.5–1000 ng/mL and LODs ranged from 0.009 to 0.11 ng/mL. The applications of the developed method demonstrated that it simultaneously determined all target BAs in different biological sample matrices with excellent sensitivity, selectivity and reproducibility.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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