| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1212370 | Journal of Chromatography B | 2014 | 5 Pages |
•Tissue based columns.•Multiple transmembrane protein stationary phases.•Membrane enclosed organelle columns.
Cellular membrane affinity chromatography stationary phases have been extensively used to characterize immobilized proteins and provide a direct measurement of multiple binding sites, including orthosteric and allosteric sites. This review will address the utilization of immobilized cellular and tissue fragments to characterize multiple transmembrane proteins co-immobilized onto a stationary phase. This approach will be illustrated by demonstrating that multiple transmembrane proteins were immobilized from cell lines and tissue fragments. In addition, the immobilization of individual compartments/organelles within a cell will be discussed and the changes in the proteins binding/kinetics based on their location.
