Article ID Journal Published Year Pages File Type
1212473 Journal of Chromatography B 2014 5 Pages PDF
Abstract

•An LC–MS/MS method has been developed for determining sitafloxacin in human plasma.•The method has some merits: high sensitivity, small sample volume and short runtime.•It is successfully applied to the pharmacokinetic study of sitafloxacin in human.

A high-performance liquid chromatographic–tandem mass spectrometric (HPLC–MS/MS) method was developed and validated to determine sitafloxacin in human plasma with dextrorphan as internal standard. Chromatographic separation was performed on a ZORBAX SB-C18 column (3.5 μm, 2.1 mm × 100 mm) with the mobile phase of methanol/water (containing 0.1% formic acid) (46:54, v/v) at a flow rate of 0.2 mL/min. Quantification was performed using multiple-reaction monitoring of the transitions at m/z 410.2 ⟶ 392.2 for sitafloxacin and m/z 258.1 ⟶ 157.1 for dextrorphan, respectively. The calibration curve was linear over the range of 5–2500 ng/mL with the lower limit of quantification of 5 ng/mL for sitafloxacin. The intra- and inter-day precisions were less than 8.3% and the deviations of assay accuracies were within ±4.1%. Sitafloxacin was sufficiently stable under all relevant analytical conditions. This method was successfully applied to the pharmacokinetic study of sitafloxacin in healthy Chinese volunteers.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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