Article ID Journal Published Year Pages File Type
1212572 Journal of Chromatography B 2014 7 Pages PDF
Abstract

•This method quantify the 7 NAEs involved in the largest number of pathology.•Method validate in large concentration range (0.125–200 ng/mL).•Method applicable to numerous matrices (plasma, serum, urine and several tissues).•Method for clinical analysis, could disclose novel diagnostic or prognostic markers.•Method suitable for pharmacological research targeting the Endocannabinoid system.

We describe and validate a sensitive UHPLC-ESI-QTOF-MS method for the simultaneous quantification of seven endocannabinoids and non-endocannabinoids related N-acylethanolamides: N-arachidonoylethanolamide, N-palmitoylethanolamide, N-stearoylethanolamide, N-oleoylethanolamide, N-linoleoylethanolamide, N-α-linolenoylethanolamide and N-eicosapentaenoylethanolamide in several bio-matrices for the purpose of research and clinical application. We examined effects of different liquid–liquid and solid phase extraction on the recovery of endocannabinoids and N-acylethanolamides. Protein precipitation with cooled acetone and extraction with acetonitrile (1% v/v formic acid) using OASIS HLB cartridge gave better results. Separation was performed on a Waters Acquity UPLC HSST3 column using a 9 min elution gradient coupled with high resolution mass spectrometry (QTOF/MS). The high sensitivity of the developed method allow its application on sample with low volumes or low levels of endocannabinoids and N-acylethanolamides and make the method suitable for routine measurement in human bio-matrices, such as plasma, serum (500 μL), urine (1 mL) and tissues (10–30 mg). Its application in clinical research could contribute to unravel pathophysiological roles of these family of lipid mediators and disclose novel diagnostic and prognostic markers.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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