Chromatographic resolution of angiotensin II receptor antagonists (sartans)

•HPLC based method for unified quantification of sartans.•Validation analyses demonstrated the method precision in the range of linearity.•High throughput HPLC method to measure multiple sartans in pharmaceutical samples.

First time a simple, sensitive and unified quantification method has been developed to analyze the complete class of angiotensin II receptor antagonists which are used in the treatment of hypertension either alone or in combination with some other drugs. The most important advantage of developed method was that the eight separate drugs can be determined on a single chromatographic system without modifications in detection wavelength and mobile phase. The drugs were separated on a Purospher Star 4.6 mm × 25 cm, 5 μm, C18 column maintained at 40 °C with 1 mL min−1 flow rate using ultra violet detection at 254 nm. Good separation (Rs > 2.0) was achieved in a short analysis allowing simultaneous determination of all eight sartans. The effect of variation in flow rate, detection wavelength and column oven temperature was also studied. The proposed method was statistically validated in terms of precision, accuracy, linearity, specificity and robustness. The newly developed method proved to be specific, robust and accurate for the quantification of eight sartans in commercial pharmaceutical formulations.