Synthesis of molecular imprinted polymers for selective extraction of domperidone from human serum using high performance liquid chromatography with fluorescence detection

•Compared with previously LLE methods, our SPE method has a faster extraction, less consumption of hazardous solvent, and no emulsion formation.•In pharmacokinetic studies that require a large number of sample analyses, the reported SPE–MIP method may be suitable due to time saving advantages.•In this paper the synthesis and using MIP for selective extraction domperidone from human serum by HPLC-fluorescence was carry out.

In this study a novel method is described for selective quantization of domperidone in biological matrices applying molecular imprinted polymers (MIPs) as a sample clean up procedure using high performance liquid chromatography coupled with a fluorescence detector. MIPs were synthesized with chloroform as the porogen, ethylene glycol dimethacrylate as the crosslinker, methacrylic acid as the monomer, and domperidone as the template molecule. The new imprinted polymer was used as a molecular sorbent for separation of domperidone from serum. Molecular recognition properties, binding capacity and selectivity of MIPs were determined. The results demonstrated exceptional affinity for domperidone in biological fluids. The domperidone analytical method using MIPs was verified according to validation parameters, such as selectivity, linearity (5–80 ng/mL, r2 = 0.9977), precision and accuracy (10–40 ng/mL, intra-day = 1.7–5.1%, inter-day = 4.5–5.9%, and accuracy 89.07–98.9%).The limit of detection (LOD) and quantization (LOQ) of domperidone was 0.0279 and 0.092 ng/mL, respectively. The simplicity and suitable validation parameters makes this a highly valuable selective bioequivalence method for domperidone analysis in human serum.