Article ID Journal Published Year Pages File Type
1212952 Journal of Chromatography B 2015 5 Pages PDF
Abstract

•A method was fully validated to determine bosutinib in rat plasma.•This is the most sensitive analysis for bosutinib.•The plasma sample was prepared by a protein precipitation.•Bosutinib was analyzed in rat plasma up to 48 h after administration.

In this work, a simple, sensitive and fast ultra performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the quantitative determination of bosutinib in rat plasma. Plasma samples were processed with a protein precipitation. The separation was achieved by an Acquity UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm) column with a gradient mobile phase consisting of 0.1% formic acid in water and acetonitrile. Detection was carried out using positive-ion electrospray tandem mass spectrometry via multiple reaction monitoring (MRM). The validated method had an excellent linearity in the range of 0.1–500 ng/mL (R2 > 0.9977) with a lower limit of quantification (0.1 ng/mL). The extraction recovery was in the range of 75.6–85.6% for bosutinib and 81.2% for pirfenidone (internal standard, IS). The intra- and inter-day precision was below 9.7% and accuracy was from −8.1% to 8.8%. No notable matrix effect and astaticism was observed for bosutinib. The method has been successfully applied to a pharmacokinetic study of bosutinib in rats for the first time, which provides the basis for the further development and application of bosutinib.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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