Ultra high performance liquid chromatography as a tool for the discovery and the analysis of biomarkers of diseases: A review

The development and use of UHPLC-based methods for the identification, validation and analysis of biomarkers for diseases is reviewed. The currents trends in types of stationary phases and modes of detection are discussed. Afterwards, examples are provided on the use of UHPLC-MS for finding novel biomarkers in samples from in vitro or in vivo animal models of human diseases, as well as in biofluid samples (mainly urine and plasma) obtained from patients. Molecular profiling and targeted analysis are considered, providing an overview of recent experimental or clinical works carried out using UHPLC analysis of compounds from various chemical classes, such as low molecular weight metabolites, hormones, lipids, peptides and proteins.

► UHPLC-MS is a highly suitable technology for the discovery of biomarkers for diseases. ► Combining RP- and HILIC-UHPLC separations gives comprehensive covering of metabolome. ► UHPLC-MS/MS methods tailored to a class of compounds allow to identify many new metabolites. ► UHPLC-MS targeted analysis allows ultra-fast and highly selective monitoring of biomarkers.