| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1213532 | Journal of Chromatography B | 2013 | 6 Pages |
A simple and sensitive HPLC–MS/MS method was developed and validated for the simultaneous determination of decitabine and valdecitabine in rat plasma. The analytes were separated on a C18 column (150 mm × 4.6 mm, 3.5 μm) and a triple-quadrupole mass spectrometer equipped with an electrospray ionization (ESI) source was applied for detection. A clean solid-phase extraction procedure with cation exchange cartridge was employed to extract the analytes from rat plasma with high recovery of decitabine (>82%). The calibration curves were linear over a concentration range of 10–10,000 ng/mL for decitabine and 5–500 ng/mL for valdecitabine. The lower limit of quantitation (LLOQ) of decitabine and valdecitabine was 10 and 5 ng/mL, respectively. The intra-day and inter-day precisions were less than 15% and the relative error (RE) was all within ±15%. The validated method was successfully applied to a pharmacokinetics study in rats after either decitabine or valdecitabine orally administrated to the Sprague-Dawley rats.
► A C18 column (150 mm × 4.6 mm, 3.5 μm) was applied to obtain satisfactory separation. ► A cation exchange solid phase extraction provided high recovery and clean sample. ► The single analysis time was short and close to 15 min. ► The LLOQ was 10 ng/mL for decitabine using only 50 μL of rat plasma. ► The method was sufficiently sensitive to measure low concentration of decitabine.
