Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1213556 | Journal of Chromatography B | 2011 | 4 Pages |
Abstract
A PEG/citrate aqueous two-phase system was tested in the partition of commercial Concanavalin A (Con A) and subsequently applied to the extraction and purification of Con A from the crude extract of Canavalia ensiformis seeds. Con A was successfully extracted to the bottom phase of a system composed of 22% (w/w) PEG8000 and 12% (w/w) citrate at pH 6.0. The obtained purification factor was 11.5 without any loss in the hemagglutinating activity. The purity of extracted lectin was confirmed by SDS-PAGE analysis.
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Authors
Paulo A.G. Soares, Cynthia O. Nascimento, Tatiana S. Porto, Maria T.S. Correia, Ana L.F. Porto, Maria G. Carneiro-da-Cunha,