One-step extraction for gas chromatography with flame photometric detection of 18 organophosphorus pesticides in Chinese medicine health wines

An easy, rapid and selective gas chromatography with flame photometric detection (GC-FPD) method was established for simultaneously determining 18 organophosphorus pesticides (OPPs) in 80 Chinese medicine (CM) health wines. This method was based on a simple one-step extraction procedure using a little solvent without any further cleanup steps. The optimized extraction solvent for the pesticides is acetone:dichloromethane (1:1, V/V) with extraction recovery of 79.0–109.1% and relative standard deviation (RSD) of 0.36–12.68%, respectively. The limits of detection (LODs) of the established GC-FPD method for all investigated pesticides ranged from 1 to 15 ng mL−1 and limits of quantification (LOQs) from 4 to 50 ng mL−1. Out of all 80 CM health wines, 18 OPPs were found in 8 samples at low concentrations of 8.2–37.9 ng mL−1. These pesticides were successfully confirmed by GC–MS. This is the first report of determining OPPs in CM health wines, providing references for monitoring the quality of CM health wine in routine analysis.

► An easy, rapid and selective GC-FPD method was established for determining 18 OPPs in Chinese medicine health wines. ► The OPPs in health wines were extracted by a simple one-step extraction procedure. ► This extraction procedure only needed a little solvent without any further cleanup steps. ► These pesticides were successfully confirmed by GC–MS.