Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1213911 | Journal of Chromatography B | 2010 | 5 Pages |
Abstract
This paper describes how distribution ratios were used for prediction of peak elution in analytical high-performance counter-current chromatography (HPCCC) to explore the method for separation and purification of bioactive compounds from the roots of Menispermum dauricum. Then important parameters related to HPCCC separations including solvent systems, sample concentration, sample loading volume and flow rate were optimized on an analytical Mini-DE HPCCC and finally linearly scaled up to a preparative Midi-DE HPCCC with nearly the same resolutions and separation time. Four phenolic alkaloids were for the first time obtained by HPCCC separation with a two-phase solvent system composed of petroleum ether-ethyl acetate-ethanol-water (1:2:1:2, v/v). This process produced 131.3Â mg daurisolin, 197.1Â mg dauricine, 32.4Â mg daurinoline and 14.7Â mg dauricicoline with the purity of 97.6%, 96.4%, 97.2% and 98.3%, respectively from 500Â mg crude extract of the roots of M. dauricum in a one-step separation. The purities of compounds were determined by high-performance liquid chromatography (HPLC). Their structures were identified by electrospray ionization mass spectrometer (ESI-MS) and nuclear magnetic resonance (NMR).
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Houding Luo, Ming Peng, Haoyu Ye, Lijuan Chen, Aihua Peng, Minghai Tang, Fan Zhang, Jie Shi,