Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1214211 | Journal of Chromatography B | 2010 | 5 Pages |
Abstract
We developed a simultaneous diagnostic method for phenylketonuria (PKU) and galactosemia through simultaneous determination of phenylalanine (Phe) and galactose (Gal) by high-performance liquid chromatography (HPLC) with pulsed amperometric detection (PAD). The intra- and inter-day precisions were <5.8%, with satisfactory mean recoveries (98.2–105%). For all PKU-positive samples, Phe levels were above the cut-off value (>30.0 mg/L), but Gal levels were nearly zero. For 77% of galactosemia-positive samples, Phe levels were above the cut-off value, but Gal levels were above the cut-off value (>80.0 mg/L) for all samples. Our HPLC-PAD method can reduce the false-positive rate of misdiagnosis for PKU and galactosemia.
Related Topics
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Authors
Nam-Hee Kim, Ji-Seon Jeong, Ha-Jeong Kwon, Yong-Moon Lee, Hye-Ran Yoon, Kyoung Ryul Lee, Seon-Pyo Hong,