| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1214306 | Journal of Chromatography B | 2009 | 6 Pages |
Abstract
Steroid derivatization was investigated by varying the experimental parameters (reagent, reaction time, and reaction temperature) to determine the optimal conditions for individual steroids, and for larger subsets. Three methods of derivatization enhancement were also investigated: the use of sonication, the use of a microwave heating, and the addition of solvents to the reaction mixture. On a comprehensive level, derivatization using N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA) was most efficient, while the application of solvent addition and microwave heating, in several cases, provided a clear enhancement. In addition, generalized rules for steroid derivatization are described.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
John A. Bowden, Dominic M. Colosi, Diana C. Mora-Montero, Timothy J. Garrett, Richard A. Yost,