Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1214344 | Journal of Chromatography B | 2010 | 6 Pages |
A method for separation and detection of major and minor components in complex mixtures has been developed, utilising two-dimensional high-performance liquid chromatography (2D-HPLC) combined with electrospray ionisation ion-trap multiple-stage mass spectrometry (ESI-ITMSn). Chromatographic conditions were matched with mass spectrometric detection to maximise the number of components that could be separated. The described procedure has proven useful to discern several hundreds of saponin components when applied to Quillaja saponaria Molina bark extracts. The discrimination of each saponin component relies on the fact that three coordinates (x, y, z) for each component can be derived from the retention time of the two chromatographic steps (x, y) and the m/z-values from the multiple-stage mass spectrometry (zn, n = 1, 2, …). Thus an improved graphical representation was obtained by combining retention times from the two-stage separation with +MS1 (z1) and the additional structural information from the second mass stage +MS2 (z2, z3) corresponding to the main fragment ions. By this approach three-dimensional plots can be made that reveal both the chromatographic and structural properties of a specific mixture which can be useful in fingerprinting of complex mixtures.