| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1214514 | Journal of Chromatography B | 2009 | 6 Pages |
Abstract
An improved liquid chromatography–mass spectrometry method for the determination of pemetrexed in human plasma was developed and validated using a simple quadrupole LC–MS and a new SPE cartridge (Plexa® Bond Elut). The analysis was achieved with a C18 analytical column using a mobile phase consisting of formic acid/acetonitrile and isocratic flow for 7 min. The linear ranges (r2 > 0.99) were found from 5 to 5000 ng/mL. The lower limit of detection was 2.5 ng/mL. Within-day and between-day precisions were less than 7.2% and inaccuracy did not exceed 2.8%. This new method is suitable to support pharmacokinetic studies and drug monitoring.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
C. Bobin-Dubigeon, M.B. Amiand, C. Herrenknecht, J.M. Bard,