| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1214691 | Journal of Chromatography B | 2011 | 5 Pages |
Abstract
A chemical method for the determination of hyaluronan (hyaluronic acid, HA) has been developed and applied to the human blood plasma. Human blood plasma HA was converted to the ΔDi-HA by digestion with hyaluronidase SD and determined by a sensitive and selective high-performance liquid chromatography (HPLC). The HPLC includes the separation and detection of ΔDi-HA using a graphitized carbon column and fluorometric reaction with 2-cyanoacetamide in an alkaline eluent. The calibration graph for ΔDi-HA was linear over the range 0.2 ng–1 μg. It was revealed that the concentration of HA in normal human blood plasma is very low levels (about 24 ng/ml) in comparison to low-sulfated chondroitin 4-sulfate (about 13 μg/ml).
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Hidenao Toyoda, Fumie Muraki, Toshio Imanari, Akiko Kinoshita-Toyoda,