Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1214804 | Journal of Chromatography B | 2009 | 8 Pages |
Abstract
The combination of liquid chromatography (LC) with mass spectrometry (MS) has become a mainstream proteome analysis strategy. In LC-MS, measured masses possess their “universal” scale derived from atomic mass tables. In contrast, the observed LC retention times (RT) are not tied to a conventional time scale, and depend on experimental conditions. However, RT data, being explicitly orthogonal to MS, offer relevant information for proteome characterization. We present here a strategy for peptides RT data standardization, based on the generation of a standard scale using retention prediction models, which enables sharing of identification databases in the context of multi-laboratory research.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
I.A. Tarasova, V. GuryÄa, M.L. Pridatchenko, A.V. Gorshkov, S. Kieffer-Jaquinod, V.V. Evreinov, C.D. Masselon, M.V. Gorshkov,