Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1214902 | Journal of Chromatography B | 2010 | 5 Pages |
Abstract
We characterized the two-dimensional electrophoretic patterns of fibrinogen chains α, β, and γ from the plasma of six animal species - Bos taurus, Canis familiaris, Equus caballus, Felis catus, Gallus domesticus and Sus scrofa. Comparing the spots resolved from serum and plasma samples, or exploiting the cross-reactivity of animal fibrinogen with an antiserum raised against the human protein could detect only some of the fibrinogen chains. Conversely, the analysis of the precipitate obtained by heating plasma for some minutes at 56 °C was adequate for the recognition of all fibrinogen chains in all samples. Physicochemical properties of the homologous proteins were found to extensively vary across species, with complete separation among the mapping areas for α, β and γ chains and maximal heterogeneity among β chains.
Keywords
SDSone-dimensional electrophoresisSerum/plasma1-DEHRPOFFP2-DECPKIgGDTT3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonateIPGimmobilized pH gradientTwo-dimensional electrophoresisimmunoglobulin Gdithiothreitolsodium dodecyl sulfateFibrinogenIsoelectric pointMolecular weightHorseradish peroxidasefresh-frozen plasmaCHAPSCreatine phosphokinaseAnimal species
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ingrid Miller, Elisabetta Gianazza, Manfred Gemeiner,