| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1214968 | Journal of Chromatography B | 2008 | 7 Pages |
Abstract
An enantioselective method using liquid-phase microextraction (LPME) followed by HPLC analysis was developed for the determination of oxybutynin (OXY) and its major metabolite N-desethyloxybutynin (DEO) in rat liver microsomal fraction. The LPME procedure was optimized using multifactorial experiments. Under the optimal extraction conditions, the mean recoveries were 61 and 55% for (R)-OXY and (S)-OXY, respectively, and 70 and 76% for (R)-DEO and (S)-DEO, respectively. The validated method was employed to an in vitro biotransformation study using rat liver microsomal fraction. The results demonstrated the enantioselective biotransformation of OXY.
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Authors
Patrícia da Fonseca, Luis Alexandre Pedro de Freitas, Luis Felipe Ribeiro Pinto, Cezar Rangel Pestana, Pierina Sueli Bonato,