| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1214995 | Journal of Chromatography B | 2008 | 7 Pages |
Abstract
This work describes an assay system that has been developed to quantify raltegravir concentrations in human plasma using a liquid–liquid extraction technique paired with HPLC separation and MS–MS detection. The dynamic range of this assay extends from 1 to 3000 ng/mL, with a coefficient of determination (r2, mean ± SD) of 0.9992 ± 0.0002. The mean precision values for calibration standards ranged from 0.6% to 3.0%, while accuracy values were 96.5–104.3%. This procedure is an accurate, precise, and sensitive method for raltegravir quantitation and was successfully validated using external proficiency testing.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Mary C. Long, Chantelle Bennetto-Hood, Edward P. Acosta,