| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1215095 | Journal of Chromatography B | 2008 | 4 Pages |
Abstract
The preparation of plasma membrane (PM) proteome samples is seriously difficult and time-consuming, owing to their profound hydrophobicity and low abundance. We have developed an efficient PM sample preparation method using Ultracentrifugation with Percoll and an aqueous two-phase extraction. The developed method was rapid (3 h) and provided high purities (26-fold of cell lysate) with a high yield (2.6% of whole cell lysate proteins). This method is especially useful for PM proteome studies using 2D gel electrophoresis.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Seung-Ah Park, Mi-Ryung Kim, Pan-Kyeom Kim, Hyung-Don Cho, Gi-Yeon Han, Chan-Wha Kim,