Article ID Journal Published Year Pages File Type
1215353 Journal of Chromatography B 2007 5 Pages PDF
Abstract

A method of indirectly measuring pyruvic acid (PA) by capillary electrophoresis with amperometric detection is proposed for the first time. It is based on the oximation reaction between PA and hydroxylamine (NH2OH), and the quantification of PA was performed by direct and sensitive amperometric detection of excessive NH2OH after the oximation reaction. This method displayed a good sensitivity, and the detection limits of NH2OH and PA are 1.76 × 10−7 and 3.88 × 10−7 mol/L, respectively at S/N = 3. The linear relationship between the peak current and PA concentration is exhibited over the range from 4 × 10−6 to 1 × 10−4 mol/L. This method has been applied to determine PA in rat plasma with satisfactory results.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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