Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1215448 | Journal of Chromatography B | 2007 | 8 Pages |
Abstract
A high throughput off-line μElution 96-well solid phase extraction (SPE) followed by liquid chromatography with tandem mass spectrometry (LC/MS/MS) quantification for the determination of N-acetyl retigabine in dog plasma has been developed and validated. The method involves the use of μElution 96-well SPE for the simultaneous extraction of N-acetyl retigabine and rapid removal of its N-glucuronide metabolite that has shown to be problematic due to its instability using other clean-up methods. The μElution SPE technology eliminates the need for post-extraction solvent evaporation and greatly reduces sample preparation time consequently improving assay efficiency.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Wei Bu, Mai Nguyen, Christine Xu, Chin-Chung Lin, Li-Tain Yeh, Virginia Borges,