| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1215480 | Journal of Chromatography B | 2007 | 5 Pages |
Abstract
A simple and sensitive HPLC method for the simultaneous analysis of free MPA and free MPAG was developed. Separation was achieved on a X-Terra RP18 column with acetonitrile–40 mM orthophosphoric acid as eluents using a gradient elution mode over 35 min at a flow rate of 1.5 ml/min. The assay was linear in the range 0.005 mg/L (LOQ) to 5 mg/L for free MPA and 0.05 mg/L (LOQ) to 200 mg/L for free MPAG. Isolation of free MPA and free MPAG was done by ultrafiltration and the ultrafiltrate was directly injected. A positive correlation between MPA free fractions and free MPAG concentrations was found. Likewise, free MPAG was related to total MPAG concentrations in the seven heart transplant patients.
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Analytical Chemistry
Authors
Xavier Cussonneau, Magali Bolon-Larger, Céline Prunet-Spano, Olivier Bastien, Roselyne Boulieu,