Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1215481 | Journal of Chromatography B | 2007 | 5 Pages |
Abstract
Preparative high-speed counter-current chromatography (HSCCC) coupled with evaporative light scattering detection (ELSD) was successfully applied to the isolation and purification of four triterpene saponins from Clematis mandshurica by one-step separation. The solvent system was composed of ethyl acetate–n-butanol–ethanol–0.05% TFA (5:10:2:20, v/v). The purities of all the saponins obtained were better than 97%. The structures of the compounds were elucidated by the means of spectroscopic methods.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Shepo Shi, Dan Jiang, Mingbo Zhao, Pengfei Tu,