Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
1215500 | Journal of Chromatography B | 2008 | 6 Pages |
Abstract
We present a fast and reproducible method for steroid analysis (corticosterone, deoxycorticosterone, progesterone, 17α-hydroxyprogesterone, 11-deoxycortisol, 21-deoxycortisol, androstenedione, testosterone, dihydrotestosterone and cortisol) in small volumes of serum and in dried blood spot samples by LC-MS/MS. No derivatisation was needed. LC separation was achieved by using an Atlantis® C18 column and water–methanol–formic acid gradient as a mobile phase and a flow rate of 250 μL/min over a run time of 6 min. Steroids were measured in MRM mode with electrospray interface (positive ion mode). Validation showed excellent precision, sensitivity, recovery and linearity with coefficients of determination r2 > 0.992.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Nils Janzen, Stefanie Sander, Michael Terhardt, Michael Peter, Johannes Sander,