| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 1215624 | Journal of Chromatography B | 2007 | 7 Pages |
Abstract
A highly sensitive isocratic capillary high-performance liquid chromatographic (HPLC) method with electrochemical detection (ED) for the simultaneous measurement of serotonin (5-hydroxytryptamine, 5-HT) and its metabolite 5-hydroxyindole-3-acetic acid (5-HIAA) in microdialysates has been developed using a 0.5 mm i.d. capillary column and a 11-nL detection cell. This method, validated on both pharmacological and analytical bases, can be performed using injection volumes as low as 1 μL. The limits of detection were 5.6 × 10−11 mol/L and 3.0 × 10−9 mol/L for 5-HT and 5-HIAA. Several applications of the present method are given on microdialysates from rodent brain and human spinal cord.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Sandrine Parrot, Laura Lambás-Señas, Sabine Sentenac, Luc Denoroy, Bernard Renaud,